Part:BBa_K3726044
BOH_1_A_LV1
This is a composite part that corresponds with a level 1 MoClo part. It is a transcriptional unit for the expression of the part “BBa_K3726012” CDS_Lv0_BOH_1_A , the first and the second two enzymes of synthetic butanol biosynthesis operon.
The part in its upstream region has a homology region “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) for homologous recombination within the genome of PCC 11801. In downstream region, the part has the connector 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct following the marburg collection standard
Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079” and by the synthetic RBS, RBS* “BBa_K3726093”, which has demonstrated its increased ribosome binding strength in Synechocystis PCC6803 and other cyanobacteria in comparison with other heterotroph-derived RBS.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal NgoMIV site found at 1494
Illegal NgoMIV site found at 1594
Illegal NgoMIV site found at 1900
Illegal AgeI site found at 594
Illegal AgeI site found at 2272 - 1000COMPATIBLE WITH RFC[1000]
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